Products related to Sequencing:
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Next Generation Sequencing & Applications
Billions of spots of tiny genetic code comprise the human genome.It was DNA sequencing technology that had revolutionized genomic research by decoding the valuable genetic information by giving the picture of an exact order of occurrence of nucleotides in a DNA. The inception of first-generation sequencing method, also called Sanger sequencing took place in 1975.The first major breakthrough of first-generation sequencing comes, when the 13 year log Human Genome Project (HGP) was completed in 2003 at a cost $3 million.With ever increasing demands of researchers and clinicians, complex genomic research require a depth of information which is however beyond the capacity of traditional DNA sequencing technologies.These research questions gaps are very well addressed by Next-generation sequencing (NGS) has filled that gap of cheaper as well as faster sequencing technology. It is just a decade old technology, but it has popularize the next-generation sequencing to high-throughput sequencing hat allow millions to trillions of observations to be made in parallel during a single instrument run.Since the introduction of these technologies, the number of applications and methods that influence the power of genome-scale sequencing has increased exponentially.Although in genome research NGS has mostly superseded conventional Sanger sequencing, it has not yet translated into routine clinical practice. The following chapter will highlight the concepts, technologies, and methods of next-generation sequencing to illustrate the breadth and depth of the applications and research areas that are driving progress in genomics.
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Connecting Camels Sequencing Cards
Connecting Camels Sequencing cards for use with Connecting Camels sets. Connecting Camels are sold separately. Contents 20 laminated colour cards graded from levels 1 to 5 and teachers notes.
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Magnetic Colour Sequencing Bugs
The Magnetic Colour Sequencing Bugs are ideal for teaching patterns and sequencing skills.Children select a work card and complete the sequence on their card by joining together the magnetic bug heads and body segments with a simple snap.The cards
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Sequencing the Primary Curriculum
This book is an introduction to the primary curriculum for all trainee and early career teachers.It shows how to sequence and plan your teaching in every curriculum subject to ensure you are offering balanced and cohesive learning opportunities that align with the National Curriculum in England. Build your subject knowledge in line with the National CurriculumSequence your teaching so that key curriculum content is threaded through all your lesson plansExplore the basics of curriculum design to support your professional development and help children to learn and remember more over time This is essential reading for trainee teachers on primary initial teacher education courses including university-based (PGCE, BEd, BA with QTS); school-based (School Direct, SCITT, Teaching Apprenticeships) routes into teaching, and early career and experienced teachers wishing to enhance their practice.
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What are the differences between Sanger DNA sequencing and NGS sequencing using a sequencer?
Sanger DNA sequencing is a traditional method that involves sequencing one DNA fragment at a time using chain-terminating dideoxynucleotides. It is a slower and more labor-intensive process compared to NGS sequencing. NGS sequencing, on the other hand, uses massively parallel sequencing technology to simultaneously sequence millions of DNA fragments. This allows for high-throughput sequencing and the generation of large amounts of data in a shorter amount of time. Additionally, NGS sequencing can provide more comprehensive and detailed information about the entire genome, making it more suitable for large-scale genomic studies.
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What is the DNA sequencing for guanine?
The DNA sequencing for guanine is represented by the letter "G". Guanine is one of the four nucleobases found in DNA, along with adenine, cytosine, and thymine. It pairs with cytosine through three hydrogen bonds in the DNA double helix structure. The specific sequence of guanine, along with the other nucleobases, forms the genetic code that determines the characteristics and functions of an organism.
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Why are modern DNA sequencing methods faster?
Modern DNA sequencing methods are faster due to advancements in technology and automation. High-throughput sequencing machines can process multiple samples simultaneously, increasing the speed of data generation. Additionally, improvements in chemistry and bioinformatics have streamlined the sequencing process, reducing the time and resources required for analysis. These advancements have made it possible to sequence large genomes in a fraction of the time it would have taken with older methods, revolutionizing the field of genomics.
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What is the difference between sequencing and transposition?
Sequencing is the process of determining the precise order of nucleotides in a DNA or RNA molecule. It involves identifying the sequence of bases (A, T, C, G) in a specific region of genetic material. Transposition, on the other hand, is a genetic process where a segment of DNA moves from one location in the genome to another. This can result in genetic mutations or changes in the expression of certain genes. In summary, sequencing involves determining the order of nucleotides, while transposition involves the movement of genetic material within the genome.
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Nanopore Sequencing: An Introduction
This is an introductory text and laboratory manual to be used primarily in undergraduate courses.It is also useful for graduate students and research scientists who require an introduction to the theory and methods of nanopore sequencing.The book has clear explanations of the principles of this emerging technology, together with instructional material written by experts that describes how to use a MinION nanopore instrument for sequencing in research or the classroom.At Harvard University the book serves as a textbook and lab manual for a university laboratory course designed to intensify the intellectual experience of incoming undergraduates while exploring biology as a field of concentration.Nanopore sequencing is an ideal topic as a path to encourage students about the range of courses they will take in Biology by pre-emptively addressing the complaint about having to take a course in Physics or Maths while majoring in Biology.The book addresses this complaint by concretely demonstrating the range of topics - from electricity to biochemistry, protein structure, molecular engineering, and informatics - that a student will have to master in subsequent courses if he or she is to become a scientist who truly understands what his or her biology instrument is measuring when investigating biological phenomena.
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Sequencing Visual Texts : Book 1
Sequencing visual texts is a series of books which provide resources to support the teaching and learning of sequencing in early years classes.The series supports pupils struggling with written texts and those who need to develop oral communication skills.The series provides background information, suggestions for additional activities, and pictorial and text resources.T_i_t_l_e_s_ _i_n_ _t_h_i_s_ _s_e_r_i_e_s_ _a_r_e_:_ _Sequencing visual texts-B_o_o_k_ _1_ _Sequencing visual texts-B_o_o_k_ _2_ _Sequencing visual texts-B_o_o_k_ _3_ _
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Next-Generation Sequencing Data Analysis
Next-generation DNA and RNA sequencing has revolutionized biology and medicine.With sequencing costs continuously dropping and our ability to generate large datasets rising, data analysis becomes more important than ever.Next-Generation Sequencing Data Analysis walks readers through next-generation sequencing (NGS) data analysis step by step for a wide range of NGS applications.For each NGS application, this book covers topics from experimental design, sample processing, sequencing strategy formulation, to sequencing read quality control, data preprocessing, read mapping or assembly, and more advanced stages that are specific to each application.Major applications include: RNA-seq: Both bulk and single cell (separate chapters) Genotyping and variant discovery through whole genome/exome sequencing Clinical sequencing and detection of actionable variants De novo genome assembly ChIP-seq to map protein-DNA interactions Epigenomics through DNA methylation sequencing Metagenome sequencing for microbiome analysis Before detailing the analytic steps for each of these applications, the book presents introductory cellular and molecular biology as a refresher mostly for data scientists, the ins and outs of widely used NGS platforms, and an overview of computing needs for NGS data management and analysis.The book concludes with a chapter on the changing landscape of NGS technologies and data analytics.The second edition of this book builds on the well-received first edition by providing updates to each chapter.Two brand new chapters have been added to meet rising data analysis demands on single-cell RNA-seq and clinical sequencing.The increasing use of long-read sequencing has also been reflected in all NGS applications.This book discusses concepts and principles that underlie each analytic step, along with software tools for implementation.It highlights key features of the tools while omitting tedious details to provide an easy-to-follow guide for practitioners in life sciences, bioinformatics, biostatistics, and data science.Tools introduced in this book are open source and freely available.
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Korg Wavestate MKII Sequencing Synthesizer
The Korg Wavestate MKII Sequencing Synthesizer is the latest edition of the now legendary Wavestate synthesizer featuring expanded polyphony (now 96 voices) a premium-grade 37-note velocity-sensitive keybed and a revamped look. Not only that but the sound library has been updated with fresh new sounds to inspire your creativity. Utilising the latest Wavestate 2.0 technology you can now explore unchartered tonal territory allowing you to create virtually any sound you can imagine. Pre-loaded with hundreds of genre-defying dynamic sounds and 120-note stereo polyphony; the Wavestate SE is perfect for everything from sound design to live performances. With a wide range of hands-on controls at your disposal you can twist turn and press your way to the sound you want with eight programmable mod knobs for maximum flexibility. You can also shape and texturise your sounds using a wide variety of onboard effects including classic Vox-style guitar amp simulations. It incorporates high-end sounds from the Kronos Krome and brand-new samples from Korg and Plugin Guru. Nearly every knob and parameter can be modulated for in-depth control. It also boasts a wide variety of classic and modern-day filters for in-depth sound design.
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Are TV and radio stations obligated to broadcast news?
TV and radio stations are not legally obligated to broadcast news, but many choose to do so as part of their commitment to serving the public interest. In some countries, there may be regulations or licensing requirements that mandate a certain amount of news programming, but this varies by jurisdiction. Ultimately, the decision to include news in their programming is up to the individual stations and their management.
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What is the difference between these two sequencing methods?
The difference between Sanger sequencing and next-generation sequencing (NGS) lies in their technology and throughput. Sanger sequencing, also known as first-generation sequencing, is a traditional method that uses chain-terminating dideoxynucleotides to sequence DNA. It is a slower and more labor-intensive process, typically used for sequencing shorter DNA fragments. On the other hand, NGS is a high-throughput method that sequences millions of DNA fragments in parallel, allowing for faster and more cost-effective sequencing of entire genomes or targeted regions. NGS also provides greater depth of coverage and can detect rare genetic variants more effectively than Sanger sequencing.
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What is the difference between DNA hybridization and DNA sequencing?
DNA hybridization is a technique used to determine the similarity between two DNA sequences by allowing them to bind together based on complementary base pairing. This method provides information on the degree of similarity between the sequences. On the other hand, DNA sequencing is a technique used to determine the exact order of nucleotides in a DNA molecule. This method provides the precise sequence of the DNA, allowing for detailed analysis of genetic information.
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Why is only a single primer used in DNA sequencing?
Only a single primer is used in DNA sequencing because the primer binds to a specific region of the DNA template, initiating the synthesis of the new DNA strand. This primer is complementary to the template DNA, allowing for the specific amplification of the target region. Using a single primer simplifies the sequencing process and ensures that only the desired region of DNA is amplified and sequenced.
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